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Yoav Gilad, Ph.D.
Research Summary
Our
research focuses on inter-primate comparisons at the sequence and
expression levels with the long-term goals of identifying genomic
regions of functional importance, understanding human gene regulatory
processes and elucidating the genetic architecture of human-specific
traits.
We are using a novel multi-species cDNA array to compare expression
levels between different primate species without the confounding effect
of sequence mismatches on hybridization intensity. Our goal is to
identify genes whose regulation in humans has evolved under natural
selection. Further experiments are aimed at understanding the mechanism
of transcription regulation (cis or trans) for individual genes or
genes involved in the same biochemical pathways.
A second project in the lab is the study of olfactory receptor (OR)
genes. We are interested in the evolution of the OR gene family in
humans, as well as in their expression and regulation, both in the
olfactory epithelium as well as in other tissues (such as testis).
Selected Papers
Gilad, Y., Oshlack, A., Smyth, G. K., Speed, T. P. and
White, P. K. Expression profiling in primates reveals a rapid evolution
of human transcription factors. Nature 2006, 440: 242-245.
Gilad, Y., Oshlack, A. and Rifkin, S. Natural selection on gene
expression. Trends in Genetics 2006, 8: 456-461
Gilad, Y. and Borevitz, J. Using microarrays to study natural
variation. Current Opinion in Genetics and Development 2006, 16: 553-558
Oshlack, A., Chabot, A. E., Smyth, G. K. and Gilad, Y. Using DNA
microarrays to study gene expression in closely related species.
Bioinformatics 2007, Advance Access published on March 23, 2007
Zhang, X., De la Cruz, O., Pinto, J. M., Nicolae, D., Firestein, S. and
Gilad, Y. Characterizing the expression of human olfactory receptor
genes. Genome Biology 2007, Advance Access published on May 17, 2007
Chabot, A. E., Shrit, R. A., Blekhman, R. and Gilad Y. Using reporter
gene assays to identify cis regulatory differences between humans and
chimpanzees. Genetics 2007, In Press.
Man, O., Willhite, D. C, Crasto, C. J., Shepherd, G.M. and Gilad, Y. A
framework for exploring functional variability in olfactory receptor
genes. PLoS One 2007, Aug 1;2:e682.
de Candia, P., Blekhman, R., Chabot, A. E., Oshlack, A. and Gilad, Y. A
direct transcriptional target of human FOXO1 mediates response to
oxidative stress. PLoS One 2008, In Press.
Marioni, J., Mason, C. E., Shrikant, M. M., Stephens, M., and Gilad, Y.
RNA-seq: An assessment of technical reproducibility and comparison with
gene expression arrays. Genome Research 2008, Jun 11. (Advanced
publication)
Reinius, B., Saetre, P., Leonard, J. A., Blekhman, B., Martinez, R.,
Gilad, Y., and Jazin, H. An evolutionarily conserved sexual signature
in the primate brain. PLoS Genetics 2008, 3: e1670.
Gilad, Y., Rifkin, S. A., and Pritchard, J. K. Revealing the
architecture of gene regulation: the promise of eQTL studies. Trends in
Genetics 2008, I24:408-415.
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