Stephen Kron, M.D., Ph.D.

Cell Cycle Checkpoints

Research Summary

The Kron laboratory is a diverse and collaborative group of cell biologists, geneticists, biochemists, chemists and computer scientists. Our current basic research and technology efforts include 1) defining roles for chromatin dynamics and cell cycle regulation in DNA damage checkpoint response and cellular senescence, 2) dissecting cross-talk between metabolism and DNA damage response, 3) developing novel molecular assays to interrogate cell signaling in cancer, and 4) implementing novel mass spectrometry approaches to enable quantitative proteomics. We also pursue translational projects directed at 1) discovering inhibitors of cellular response to DNA double strand breaks as an approach to radiosensitization, 2) examining DNA damage and repair in tissues and tumors, and 3) exploiting DNA damage responses to induce anti-tumor immune responses.

Selected Papers

A.W. Truman, K. Kristjansdottir, D. Wolfgeher, N. Hasin, S. Polier, H. Zang, S. Perrett, C. Prodromou, G.W. Jones and S.J. Kron, "CDK-dependent Hsp70 phosphorylation controls G1 cyclin abundance and cell cycle progression," Cell, 151:1308-18, 2012 (PubMed)

G. Zhou, F. Khan, Q. Dai, J.E. Sylvester, and S.J. Kron, "Photocleavable peptide-oligonucleotide conjugates for protein kinase assays by MALDI-TOF MS," Molecular Biosystems, 8:2395-404, 2012 (PubMed)

J.E. Sylvester, T.S. Bray and S.J. Kron, "Annotator: Post-processing software for generating function-based signatures from quantitative mass spectrometry," J. Proteome Res. 11:1521-36, 2012 (PubMed)

Y. Meng, E.V. Efimova, K.W. Hamzeh, T.E. Darga, H.J. Mauceri, Y.-X. Fu, S.J. Kron and R.R. Weichselbaum, "Radiation-inducible immunotherapy for cancer: Senescent tumor cells as a cancer vaccine," Mol Ther. 20:1046-55, 2012 (PubMed)

E. Labay, E.V. Efimova, B.K. Quarshie, D.W. Golden, R.R. Weichselbaum, and S.J. Kron, "Ionizing Radiation Induced Foci (IRIF) persistence screen to discover enhancers of accelerated senescence," Int. J. High Throughput Screening, 2011(2):1-13, 2011 (Dovepress)

O.A. Ulanovskaya, J. Cui, S.J. Kron and S.A. Kozmin, "A pairwise chemical genetic screen identifies new inhibitors of glucose transport," Chemistry & Biology, 18:222-30, 2011
(PubMed)

G. Zhou, J.E. Sylvester, D. Wu, D.R. Veach and S.J. Kron, "A magnetic bead-based protein kinase assay with dual detection techniques," Anal. Biochem., 408:5-11, 2011 (PubMed)

D. Rosetto, A.W. Truman, S.J. Kron and J. Côté, "Epigenetic modifications in double-strand break DNA damage signaling and repair," Clin. Cancer Res., 16:4543-52, 2010 (Clin. Cancer Research)

K. Kristjansdottir and S.J. Kron, "Stable-isotope labeling for protein quantitation by mass spectrometry," Current Proteomics, 7:144-155, 2010 (Current Proteomics)

G. Zhou, X. Yan, D. Wu and S.J. Kron, "Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS," Bioconj. Chem., 21:1917-24, 2010 (PubMed)

M.R. Mand, D. Wu, D.R. Veach and S.J. Kron, "Cell treatment and lysis in 96 well filter-bottom plates for screening Bcr-Abl activity and inhibition in whole cell extracts," J. Biomol. Screening, 15:434-40, 2010 (PubMed)

J.E. Sylvester and S.J. Kron, "A bead-based activity screen for small-molecule inhibitors of signal transduction in chronic myelogenous leukemia cells," Mol. Cancer Ther., 9:1469-81, 2010(PubMed)

D. Wu, J.E. Sylvester, L.L. Parker, G. Zhou and S.J. Kron, "Peptide reporters of kinase activity in whole cell lysates," Biopolymers: Peptide Science, 94:475-86, 2010 (PubMed)

E.V. Efimova, H.J. Mauceri, D.W. Golden, E. Labay, V.P. Bindokas, T.E. Darga, C. Chakraborty, J.C.B. Andrade, C. Crawley, H.G. Sutton, S.J. Kron and R.R. Weichselbaum, "Poly(ADP-ribose) polymerase inhibitor induces accelerated senescence in irradiated breast cancer cells and tumors," Cancer Res., 70:6277-82, 2010 (PubMed)